Dear cell culture users,
We are happy to announce that the qPCR-based mycoplasma detection service will be offered again on the first Wednesday of every month starting from October, just as before.
Key features of our detection protocol include (https://labsupport.pages.ist.ac.at/mycoplasma-detection/):
- Real-time PCR technology for rapid quantitative and qualitative analysis with high sensitivity
- Multiplex primers and probes targeting conserved mycoplasma DNA sequences
- Internal controls to ensure assay accuracy and sample quality
- Direct use of culture supernatant, no DNA extraction required
Please find below the sample preparation instructions (also available online here: https://labsupport.pages.ist.ac.at/mycoplasma-detection-guidelines/)
- Cultivate cells without antibiotics for at least one week.
- Collect 100 µL of supernatant from a culture at 90-100% confluence (grown for 2-3 days in the same media).
- Heat-inactivate the supernatant at 95°C for 5 minutes. Note: Samples are stable at 4°C for up to 1 week.
- Deliver samples to the 4°C fridge at the Sunstone Building, 1st Floor (I23.01.038). Tubes should be clearly labeled with user initials, sample number, and date (e.g., FS01_1oct25, FS02_1oct25). Printed labels are preferred.
- Create a PPMS order, this triggers an automated notification to us.
Pricing details are available here (login required): https://labsupport.pages.ist.ac.at/mycoplasma-detection-pricing-internal-rates/ and below:
Dear cell culture users,
We are happy to announce that the qPCR-based mycoplasma detection service will be offered again on the first Wednesday of every month starting from October, just as before.
Key features of our detection protocol include (https://labsupport.pages.ist.ac.at/mycoplasma-detection/):
- Real-time PCR technology for rapid quantitative and qualitative analysis with high sensitivity
- Multiplex primers and probes targeting conserved mycoplasma DNA sequences
- Internal controls to ensure assay accuracy and sample quality
- Direct use of culture supernatant, no DNA extraction required
Please find below the sample preparation instructions (also available online here: https://labsupport.pages.ist.ac.at/mycoplasma-detection-guidelines/)
- Cultivate cells without antibiotics for at least one week.
- Collect 100 µL of supernatant from a culture at 90-100% confluence (grown for 2-3 days in the same media).
- Heat-inactivate the supernatant at 95°C for 5 minutes. Note: Samples are stable at 4°C for up to 1 week.
- Deliver samples to the 4°C fridge at the Sunstone Building, 1st Floor (I23.01.038). Tubes should be clearly labeled with user initials, sample number, and date (e.g., FS01_1oct25, FS02_1oct25). Printed labels are preferred.
- Create a PPMS order, this triggers an automated notification to us.
Pricing details are available here (login required): https://labsupport.pages.ist.ac.at/mycoplasma-detection-pricing-internal-rates/ and below:
Register here
Dear cell culture users,
We are happy to announce that the qPCR-based mycoplasma detection service will be offered again on the first Wednesday of every month starting from October, just as before.
Key features of our detection protocol include (https://labsupport.pages.ist.ac.at/mycoplasma-detection/):
- Real-time PCR technology for rapid quantitative and qualitative analysis with high sensitivity
- Multiplex primers and probes targeting conserved mycoplasma DNA sequences
- Internal controls to ensure assay accuracy and sample quality
- Direct use of culture supernatant, no DNA extraction required
Please find below the sample preparation instructions (also available online here: https://labsupport.pages.ist.ac.at/mycoplasma-detection-guidelines/)
- Cultivate cells without antibiotics for at least one week.
- Collect 100 µL of supernatant from a culture at 90-100% confluence (grown for 2-3 days in the same media).
- Heat-inactivate the supernatant at 95°C for 5 minutes. Note: Samples are stable at 4°C for up to 1 week.
- Deliver samples to the 4°C fridge at the Sunstone Building, 1st Floor (I23.01.038). Tubes should be clearly labeled with user initials, sample number, and date (e.g., FS01_1oct25, FS02_1oct25). Printed labels are preferred.
- Create a PPMS order, this triggers an automated notification to us.
Pricing details are available here (login required): https://labsupport.pages.ist.ac.at/mycoplasma-detection-pricing-internal-rates/ and below: